Available Programs:
8
Program name:
Program name:
Program name:
Program name:
Program name:
Program name:
Program name:
Program name:
Details of the selected program:
Program name:
PRO-14W-2COL
Number of rabbits:
1
Duration
Total duration:
14 weeks
First affinity purified test antibodies:
after 6.5 weeks
Program extension:
Protein antigen analysis & optimisation
Sequence check for antigenicity:
included
Sequence check for evoultionary conservation:
included
Protein coupling to a carrier protein:
Immunization
Immunizations per rabbit:
3
Additional immunizations:
Intradermal injections at multiple sites (instead of s.c. at a single site)
included
Advanced highly efficient adjuvant:
included
Serum Collection
Pre-immune serum (5 ml):
included
Test serum (per rabbit):
5 ml
Additional test sera:
Number of regular bleeds per rabbit (~15 ml serum, each):
3 (plus exsanguination; see below)
Additional regular bleed(s) (~15 ml serum, each)
Exsanguination (>35-45 ml serum per rabbit):
included
Affinity matrix / matrices:
Synthesis of a protein affinity matrix (~2 ml NHS sepharose):
2 different matrices included (e.g. with tag and tagged protein of interest, respectively)
Synthesis of a protein affinity matrix (~4-5 ml NHS sepharose):
option (surcharge)
Fast affinity purification of 5 ml (test serum) or 15-20 ml (large bleed) per rabbit:
included
Depletion of unwanted ABs on a second column:
included
Large Scale Affinity Purifications
Affinity purification of 50 ml serum:
included
Large Scale (50 ml serum): Depletion of unwanted ABs on additional column(s):
included
Large Scale (50 ml serum): Additional depletion (twofold) of unwanted ABs on a second column:
included
Ultrafiltration & dialysis against PBS or a buffer of choice:
included
Support
Conceptual consulting (which sequence to express? Which tags? Which test system? ...):
included
Evaluation of test results based on submitted scans/photos:
explicitly encouraged, included
As flexible as possible:
Except for the guarantee packages, you can modify any antibody program or change it to any other program of the same category just by adding or subtracting individual modules or services (see 'OPTIONS program' and affinity purification options). This can be done upon short notice at any time during the ongoing project for any upcoming steps.