Article #:
0092-05
Antigen/Product:
Activin Receptor IIB, extracellular domain
Synonym(s):
ActR IIB
Uni-/SwissProt #:
Clone / Name:
ActRIIB
Host Species:
rabbit
Mono-/polyclonal:
polyclonal (pAB) | anti-(partial sequence)
Purity:
affinity purified on the antigen
Quantity (mass):
50 µg
Quantity (vol.):
100 µl
Price (net):
266.00 €
Cross-reactivity:
human, mouse, others not tested
Applications:
WB - Western blot / immunoblot, ELISA
Sugg. dilutions:
WB: 1:5,000 (0.1 µg/ml) detection limit below 1 ng ActR-IIB ecd; ELISA: 1:400 (1.25 µg/ml)
Remarks:
IG-510 specifically detects ActR-IIB extracellular domain (ecd) in mouse and human [lane1], not reactive with the related human proteins ActR-IIecd [lane 2], BMPR-IIecd [lane 3], BMPR-IAecd [lane 4], BMPR-IBecd [lane 5], ActR-Iecd [lane 6]. Upper panel: SDS-PAGE under non-reducing conditions, lower panel: SDS-PAGE in the presence of beta-mercapto ethanol
Assay conditions:
50 ng ecd per lane
Anti-ActR-IIB antibody 1:5,000 (100 ng/ml)
2nd AB: goat-anti-rabbit x HRP, ECL detection
IG-510 detects less than 1 ng of ActR-IIB ecd. Western blots of 0.1 to 20 ng Actr-IIB ecd per lane. Upper panel: SDS-PAGE under non-reducing conditions, lower panel: SDS-PAGE in the presence of beta-mercapto ethanol
Assay conditions:
0.1 to 20 ng ecd per lane
Anti-ActR-IIB antibody 1:5,000 (100 ng/ml)
2nd AB: goat-anti-rabbit x HRP, ECL detection
IG-510 specifically detects human ActR-IIB transfected into HEK 293 T cells. Different numbers of cells transfected either with the empty pCMV vector (control, left) or pCMV[ActR-IIB] (right) were analysed by Western blotting with IG-510 antibody 24 h after transfection.The antibody specifically detects the transfected protein and some degradation products, while there is no background in cells transfected with the empty control vector.
Assay conditions:
10,000 / 3,000 / 1,000 cells per lane
Anti-ActR-IIB antibody 1:5,000 (100 ng/ml)
2nd AB: goat-anti-rabbit x HRP, ECL detection
Form:
sterile filtered liquid, with sodium azide, stabilized with carrier, also available in carrier-free form (pls. enquire)
Immunization:
recombinant protein (partial sequence)
Epitope:
extracellular domain of mouse ActR-IIB
Storage:
-20°C
Shipping:
RT - ambient temperature
Availability:
in stock
Publications referring to this product:
1.
Kotzsch, A., Nickel, J., Sebald, W. & Mueller, T. D. Purification, crystallization and preliminary data analysis of ligand-receptor complexes of growth and differentiation factor 5 (GDF5) and BMP receptor IB (BRIB). Acta Crystallogr Sect F Struct Biol Cryst Commun 65, 779–783 (2009).
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