Available Programs:
6
Program name:
Program name:
Program name:
Program name:
Program name:
Program name:
Details of the selected program:
Program name:
PEP-GUARANTEED-1MG
Number of rabbits:
2
Duration
Total duration:
~14 weeks
First affinity purified test antibodies:
6.5 weeks after first immunization (i.e. ~9-10 weeks after peptide order)
Program extension:
included
Peptide(s):
Peptide selection (epitope prediction):
included
Peptide synthesis (for immunization & column; ≥15 mg, ≥80% pure)
up to 15-mer included; longer peptides with minor surcharge
N-terminal acetylation and/or C-terminal amidation:
included
Mass spectrum & HPLC diagram:
included
Peptide coupling (5 mg) to a carrier protein:
included
Protein antigen analysis & optimisation
Sequence check for antigenicity:
included
Sequence check for evoultionary conservation:
included
Protein coupling to a carrier protein:
additional/mandatory (difficult sequences only)
Immunization
Immunizations per rabbit:
minimum of 3, as many as required, all included
Additional immunizations:
included
Intradermal injections at multiple sites (instead of s.c. at a single site)
included
Advanced highly efficient adjuvant:
included
Serum Collection
Pre-immune serum (5 ml):
included
Test serum (per rabbit):
5 ml
Additional test sera:
included
Affinity matrix / matrices:
Synthesis of a peptide affinity matrix (5 mg of peptide):
included
Affinity Purification
Fast affinity purification of 5 ml (test serum) or 15-20 ml (large bleed) per rabbit:
included, total of two test sera (1/rabbit)
Large Scale Affinity Purifications
Ultrafiltration & dialysis against PBS or a buffer of choice:
included
Guarantee
Antibodies reactive with the antigen used for immunization:
guaranteed yield: 1 mg affinity purified antibody from the animal of choice, minimum
Support
Conceptual consulting (which sequence to express? Which tags? Which test system? ...):
included
Evaluation of test results based on submitted scans/photos:
explicitly encouraged, included
As flexible as possible:
Except for the guarantee packages, you can modify any antibody program or change it to any other program of the same category just by adding or subtracting individual modules or services (see 'OPTIONS program' and affinity purification options). This can be done upon short notice at any time during the ongoing project for any upcoming steps.