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immunoGlobe®Antibody Services
- immunoGlobe® is dedicated to supply customers with affordable high quality customized antibodies for their specific needs.
- Antibodies against recombinant proteins or proteins purified from natural sources:
- Please consult with us before you start a purification project: We may help you with some advise, for instance what fragment to choose or how to deal with insoluble antigens (inclusion bodies) without the need of elaborated solubilization procedures. Ask us for our proprietary protocol! You provide us with your antigen of interest, we will do the rest:
- Coupling to carrier proteins (if required, e.g. with incomplete or poor antigens) and coupling to affinity matrices (for affinity purification)
- Raising antibodies either according to our standard protocol or according to your specifications
- Affinity purification of your antibodies on one or multiple antigen cartridge(s) (even with "insoluble" antigens"!)
- Antibodies against synthetic peptides:
- When the natural protein is not available, a small peptide derived from its amino acid sequence may serve as substitute to raise an antibody against. Please consult with us before you have your own peptide synthesized:
- Even with modern tools choosing the right peptide sequence is an art! This is, why even educated peptide selections often fail to elicit antibodies that will not only bind the peptide but will also recognize the natural antigen. Did you know, that most polyclonal sera against linear epitopes will be confined to 1-3 short sequences even when the total protein was used as an antigen? Then you might appreciate the task of making a suitable antigenic peptide prediction ... !
- All our peptides are at least 80% pure and verified by mass spectrometry, as antibodies shouldn't be directed against protective groups, other detritus, and irrelevant sequences....
- As a standard, affinity purification of the test sera on an antigen cartridge is included in all our anti-peptide antibody services at no extra costs! Testing crude anti-peptide sera on complex mixtures (e.g. immunoblots of tissue homogenates) mostly is an unsatisfying job, because you can hardly discriminate specific from similar sized unspecific bands. However, with an affinity purified antibody you can tell immediately: Does the antibody recognize the natural protein ? / What is the titer (µg antibody per ml of serum) ? / Is there a specific (!) cross-reactivity with unrelated proteins containing similar sequence motifs ?
- SPECIAL Service: Antibodies against point mutations / isoforms / modifications ....
- Features that typically have been associated with monoclonal antibodies only, now are also possible with polyclonal rabbit antibodies - due to sophisticated immunization, purification, and depletion schemes on multiple proteins or peptides. Please consult with us if you have a similar project in mind that you think might not be feasible:
- antibodies specifically recognizing a neo-epitope (e.g. a cleavage site) - but not the parental protein
- antibodies discriminating between different isoforms, splicing variants, or mutants that differ in as few as one single (1) amino acid
- antibodies discriminating between different states of neighboring double-phosphorylation sites
Our philosphy is that one of the most important prerequisites for running a successful R&D service project is a permanent and direct dialog with our customers. This is required for both, the coordination of our customers' requirements with available state of the art technology and also for a timely feedback. This is, why immunoGlobe® services are available only via direct contracting and not via resellers.
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